Stock Option Grant for Michael Houghton

This filing details a grant of 8,000 stock options to Michael Houghton, a director at ASSEMBLY BIOSCIENCES, INC. (ASMB). The options, exercisable for Common Stock, were granted on June 4, 2026, with an exercise price of $24.11. These options vest on the earlier of June 4, 2027, or the Issuer's 2027 annual meeting of stockholders, assuming continuous service. The options have an expiration date of June 4, 2036. This transaction is reported on Form 4.