Officer and Director Stock Option Grant

Gina Consylman, a director at ASSEMBLY BIOSCIENCES, INC. (ASMB), was granted 8,000 stock options on June 4, 2026. These options have an exercise price of $24.11 and will vest on the earlier of June 4, 2027, or the Issuer's 2027 annual meeting of stockholders. The options expire on June 4, 2036. This filing, a Form 4, indicates a change in beneficial ownership, specifically the acquisition of derivative securities. The underlying security is Common Stock. Ms. Consylman has direct ownership of these options, and following the grant, she directly owns 8,000 shares of Common Stock underlying these options.