Amendment No. 27 to Schedule 13D for OPKO Health, Inc.

Amendment No. 27 to Schedule 13D for OPKO Health, Inc. discloses changes in the beneficial ownership of the company's common stock by The Frost Group, LLC, Frost Gamma Investments Trust, Frost Nevada Investments Trust, and Phillip Frost, M.D. The amendment highlights that Gamma Trust directly owns 214,236,448 shares (27.9% of outstanding shares), Frost Nevada owns 30,127,177 shares (3.9%), and Dr. Frost, as the sole trustee of both trusts, may be deemed to beneficially own these shares. Additionally, Dr. Frost individually owns 3,068,951 shares and has options to acquire 2,600,000 shares. The total shares beneficially owned by Dr. Frost constitute approximately 32.8% of the outstanding shares. The amendment also notes recent transactions, including Gamma Trust's acquisition of 675,000 shares on August 8, 2025, and a gift of 440,000 shares on August 18, 2025.