Amendment No. 12 to Schedule 13D by Ainos, Inc.

Ainos, Inc. filed Amendment No. 12 to Schedule 13D, disclosing changes in ownership and transactions involving its common stock. The amendment includes details of a one-for-five reverse stock split effected on June 30, 2025, and the sale of 62,794 shares of common stock by Taiwan Carbon Nano Technology Corporation (TCNT) from June 25, 2025, through October 6, 2025. Ainos KY, a Cayman Islands corporation, holds 491,263 shares directly and has voting agreements with various parties, including TCNT, ASE Test, Inc., and others, totaling 3,259,854 shares. The amendment also updates the percentage of beneficial ownership to 9.13% of the outstanding common stock. The filing indicates that Ainos KY acquired the common stock for investment purposes, while TCNT sold shares for general operating purposes. The amendment does not disclose any new contracts, arrangements, or understandings among the parties.