Initial Statement of Beneficial Ownership

Erika P. Highland, Executive Vice President, has filed an initial statement of beneficial ownership on March 30, 2026, for shares of Gladstone Investment Corporation (GAIN). This filing, designated as Document Type 3, indicates that Highland does not currently own any securities directly. The reporting period for this statement was March 20, 2026. The filing was made through Michael LiCalsi, acting as Attorney in Fact.