Power of Attorney Filing for Andrew Craig Miller

This filing relates to a Power of Attorney granted by Andrew Craig Miller, a director of Alto Neuroscience, Inc., to Amit Etkin, Nicholas C. Smith, and Erin R. McQuade. The attorneys-in-fact are authorized to execute and file SEC Forms 3, 4, and 5 on behalf of Mr. Miller, concerning his beneficial ownership and transactions in the company's securities, in accordance with Section 16 of the Securities Exchange Act of 1934. The power of attorney remains effective until Mr. Miller is no longer required to file these forms, or until revoked. The filing is dated May 29, 2026, with the power of attorney document itself dated May 26, 2026.